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KMID : 1025520000420030253
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Journal of Animal Science and Technology
2000 Volume.42 No. 3 p.253 ~ p.260
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Comparison of the Genotypes and Frequencies of MSH Receptor(MC1R) Gene in Korean Cattle , Cheju Native Black Cattle , Japanese Black and Japanese Brown Cattle
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Abstract
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PCR-RFLP analysis of MSH receptor (melanocyte-stimulating hormone receptor; MCIR) gene was carried out to confirm the genotypes and frequencies in Korean Cattle, Cheju Native Black Cattle, Japnaese Black, Japanese Brown, Holstein and Angus. Genetic relationship of these breeds and coat colors to alleles at the Extension locus, that encodes MCIR were also determined. Allele E^D E^+ and a were detected by digestion with BsrF I (or Msp I ) and Aci I . A Dominant allele E^D, which is considered to be typical allele for dominant black coat color in cattle breeds, such as Holstein and Angus, was not detected in Korean Cattle and Japnases Brown with yellowish-brown coat color. Among sixty-two Cheju Native Black Cattle and fifteen Japanese Black, only ten and eleven heads had the dominant allele E? respectively. The remaining animals possessed the genotype E^+/E^+ or E^+/e, which probably reflect alleles at the A-locus which are supposed to give rise to the recessive black color. Several animals of Cheju Native Black Cattle and Japanese Black possessed the genotype E^D/-, probably reflecting the genetic introgression of E^D allele into these breeds during the crossbreeding period for improving. These results show that the difference of MCIR genotype and frequency among these breeds may be useful for conservation of endangered Cheju native Black Cattle and also genetically discreminating between meat of Korean Cattle and that of Holstein
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